SEAWATER TEMPERATURE CHANGE RESPONSIVE GENES IN SCLERONEPHTHYA GRACILLIMUM AND THE METHOD FOR DIAGNOSING MARINE ECOSYSTEM USING THE SAME

摘要

PURPOSE: A gene of Scleronephthya gracillimum which responds to water temperature change and a method for diagnosing marine ecosystem using the same are provided to check stress on marine ecosystem. CONSTITUTION: A microarray chip for diagnosing stress of marine ecosystem comprises: entire nucleic acid sequence of Scleronephthya gracillimum-derived gene, oligonucleotide which is a fragment thereof or complementary strand molecule thereof. A method for diagnosing stress of marine ecosystem comprises: a step of isolating each RNA from Scleronephthya gracillimum of an experimental group and Scleronephthya gracillimum as a control group; a step of synthesizing the RNAs into cDNA and labeling with different fluorescence; a step of hybridizing the cDNA on a microarray chip; a step of analyzing the reacted microarray chip; and a step of comparing the expression level with the control group.