中国蛤蜊（Mactra chinensis ）是一种重要的经济贝类，分布于我国的辽宁和山东。近年来，生境恶化和过度捕捞导致我国蛤蜊资源量减少。我们利用 Illumina Hiseq－2500平台对中国蛤蜊鳃的 microRNA 转录组进行测序，运用差异表达寻找对镉刺激有响应的功能 microRNA。结果显示对照组获得了14415256条 clean reads，实验组获得了15570111条 clean reads。在这些 reads 中一共存在14584077小 RNA，包括1898035条 unique 小 RNA，其中对照组和实验组共有187859条 unique 小 RNA。两个组的小 RNA 文库的片段长度分布是相似的，大部分小 RNA 长度分布在26～27 nt。在对照组文库中最丰富的片段长度是27 nt，其次是28 nt、26 nt 和23 nt。在实验组文库中，数量最丰富的片段长度是26 nt，其次是27 nt、28 nt 和23 nt。经过对序列前体以及结构特征的分析，发现这两个组中一共有50个 microRNA，其中已知的是38个，新发现的是12个。对照组和实验组 microRNA 含量最丰富的片段长度是23 nt。通过差异表达分析，5个 microRNA 基因具有显著性差异且从对照组到实验组的表达量是上调的，其余45个没有显著性的差异。把这50个序列与中国蛤蜊转录组进行比对，一共找到了542个靶基因。5个具有表达差异的基因一共比对到11个靶基因。把这11个靶基因与 NCBI 数据库比对，4个靶基因在 COG 中得到注释，1个靶基因在 GO 中得到注释，6个靶基因在 KEGG 中得到注释，11个靶基因在 nr 数据库中得到注释，注释到的基因有泛素蛋白连接酶 E3，Wnt 信号通路，G 蛋白信号调控等。%The Chinese surf clam (Mactra chinensis )is an economically important clam,distributed in Lia-oning Province and Shandong Province.In recently years,because of coastal environmental deterioration and overfishing,the natural population of M.chinensis have considerably declined.In this paper,we stud-ied the microRNA transcriptome of gills,and control and experimental group were sequenced through Illu-mina Hiseq 2500 CE.The differential expression andlysis was used to find the functional microRNA re-sponse to the Cd2 + exposure.Through Illumina Hi-seq 2500 CE,a total of 14 415 256 clean reads and 15 570 111 clean reads were yielded in the gill of control and experimental group respectively.A total of 14 584 077 small RNA,including 187 859 unique small RNA were yielded.The distribution of the small RNA length in the two library was similar,most of them were 26 -27 nt.27 nt was the most abundant length in control group,followded by 28 nt,26 nt,and 23 nt;26 nt was the most abundant length in ex-perimental group,and followed by 27 nt,28 nt and 23 nt.50 microRNA was found in unique small RNA, including 38 conserved and 12 novel genes.The most abundant length of microRNA in the two library was the same,23 nt.Through the analyze of differential expression analysis,the expression of 5 microRNA was induced with significantly difference,other 45 microRNA was regulated up or down without signifi-cantly difference.542 target genes were yielded when the 50 microRNA were hit to mRNA genome.And the target genes of differential expression microRNA were annotated by hitting to the NCBI database,and 4 genes hit to the COG,1 genes hit to the GO,5 genes hit to the KEGG and 11 genes hit to the nr data-base.The genes hit to the NCBI database included E3 ubiquitin-protein ligase,Wnt signaling pathway and Regulator of G-protein signaling 22.
展开▼
