印度洋红树林沉积物可培养海洋放线菌多样性及其活性

摘要

[目的]本研究旨在了解印度洋红树林沉积物可培养海洋放线菌多样性、抗菌活性及产酶活性.[方法]选用24种碳源为唯一能源培养基,利用稀释平板涂布方法对8个印度洋红树林沉积物样品进行分离,并基于16S rRNA基因系统发育分析的方法研究样品中海洋放线菌多样性；对分离得到的菌株进行抗菌活性和产酶活性检测.[结果]24种唯一碳源分离培养基中,非糖类碳源特别是甘油、丙氨酸分离效果最好,其次是多糖物质,最后是单糖.共分离得到521株海洋放线菌,经并菌后选取其中的139株代表性菌株测序,结果发现它们主要分布在放线菌纲7个亚目10个科的16个属,其中35个为潜在新种.有43.1％、33.3％、26.9％、25.5％、15.7％的实验菌株分别对枯草芽孢杆菌、白色念珠菌、大肠埃希氏菌、金黄色葡萄球菌、黑曲霉具有抑制作用；有36.5％、26.5％、22.4％、15.9％的实验菌株分别具有蛋白酶活性、纤维素酶活、淀粉酶活性、酯酶活性.[结论]印度洋红树林沉积物蕴藏着丰富的海洋放线菌资源,并具有较高生物活性,为后续工作提供良好的实验材料.%[Objective] In order to explore the diversity, antimicrobial activity and enzyme-producing activity of marine actinobacteria isolated from mangrove sediments in Indian Ocean. [Methods] Eight sediments collected from mangrove sediments in Indian Ocean were treated by the plate dilution method and spread on 24 isolation media only containing sole carbon source for energy. Marine actinobacteria were isolated and identified by 16S rRNA gene sequence analysis. The antimicrobial activity and enzyme-producing activity of isolated strains were further detected by spot planting method [Results] In total 139 representative strains were selected from 521 isolates, and they were further sequenced and performed phylogenetic analysis based on their 16S rRNA gene sequences. There were 35 strains identified as potential novel species. Antimicrobial activity was detected in Bacillus subtilis, Candida albicans, Escherichia coli, Staphylococcus aureus, Aspergillus niger. Enzyme-producing activity for protease cellulase, amylase and esterase were 36. 5% , 26. 5% , 22. 4% and 15. 9% , respectively. [ Conclusion] Diverse marine actinobacteria were discovered in mangrove sediment in Indian Ocean, which have antimicrobial and enzyme activity.