大鼠肝肠联合移植对移植肠内NOS阳性神经和VIP能神经的影响

摘要

Using NADPH-diaphorase histochemical and immunohistochemical techniques, the changes in nitric oxide synthase (NOS) activities and vasoactive intestinal polypeptide (VIP) immunoreactivities in intestinal grafts after combined liver-intestine transplantation were observed. The results showed that after combined liver-intestine transplantation, the survival rate of the recipients was increased, the rejection alleviated or delayed and NOS activities and VIP immunoreactivities in the small bowel grafts exhibited well. Within metaphase to late time of transplantation, NOS activities and VIP immunoreactivities was almost normal. These results suggested that the integration of enteric nerves was important for the survival of the recipients.%应用还原型辅酶Ⅱ&黄递酶(NADPH-d)组织化学和血管活性肠肽(VIP)免疫组织化学显色法,观察大鼠肝肠联合移植后对移植肠内一氧化氮合酶(NOS)阳性神经和VIP能神经的影响.结果发现:肝肠联合移植除能提高受体动物的存活率外,还能减轻和延迟免疫排斥反应的产生,而且移植肠内NOS和VIP免疫活性反应较好,于中、晚期(7～14 d)的移植肠内,NOS活性和VIP免疫活性几乎接近正常水平.提示肠内在神经成分的完好对移植动物的存活有重要的作用.