p27kip1基因转染增强放射抗拒人食管癌细胞的放射敏感性

摘要

Objective To study the radiation-sensitizing effect of adenovirus-mediated p27kipl gene transfection in human radioresistant esophageal carcinoma cells. Methods In adenovirus-mediated p27kipl gene-transfected human radioresistant esophageal carcinoma cells,the expression of p27kipl was detected by using immunocytochemistry. The cell cycle distribution was analyzed by flow cytometry. The radiosensitivity of human esophageal radioresistant carcinoma cells was measured by standard colony formation assays. Results A radioresistant esophageal cancer cell line EC9706R was successfully established. EC9706R cells showed more obvious radiation resistance than their pro-cells(P＜0.05 ). After transfection with Ad-p27kipl , the expression of p27kipl was up-regulated in EC9706R cells. Cell cycle was arrested in G0/G1, phase in EC9706R cells transfected with p27kipl gene. In EC9706R cells transfected with p27kipl gene, their SF2 = 49.7 ％ , Dq= 1.03Gy, but in EC9706R cells SF2 = 67.4 ％, Dq=1.66Gy,the sensitization enhancement ratios of SF2 ,Dq in two types of cells before and after transfection were 1.36 and 1.60 respectively. The SF2 and Dq values in EC9706R cells transfected with p27kipl gene were obviously lower than in EC9706R cells,and the difference in terms of SF2 , Dq , SERSF2 and SERDq was all significant( P＜0.05) . Conclusion Adenovirus-mediated p27 kipl gene transfection may enhance radiosensitivity of human radioresistant esophageal carcinoma cells.%目的 研究腺病毒介导的p27kip1基因转染对人放射抗拒食管癌细胞放射敏感性的影响.方法 以重组腺病毒介导的p27kip1基因转染人放射抗拒食管癌细胞EC9706R,免疫细胞化学法检测p27kip1表达;流式细胞仪检测转染前后肿瘤细胞周期,克隆形成实验检测p27kip1基因转染前后两种EC9706R细胞的放射敏感性.结果 腺病毒转染后放射抗拒食管癌EC9706R细胞p27kip1表达明显升高,细胞周期结果显示G0/G1期阻滞;转染p27kip1前后放射敏感性参数检测结果比较:转染前EC9706R细胞SF2=67.4%,Dq=1.66 Gy,转染后SF2=49.7%,Dq=1.03 Gy,放射增敏比SERSF2、SERDq分别为1.36,1.61,两者比较差异有统计学意义(P＜0.05).结论 p27kip1基因转染能增强人放射抗拒食管癌细胞的放射敏感性.