Fe3 O4-壳聚糖-胶原-纳米羟基磷灰石原位复合r支架的仿生制备及表征

摘要

In order to prepare hybrid nanostructure composite with magnetocaloric effect,combining with the su-perior characteristics of chitosan (CS)and collagen (Col)as the organic matrix,the soluble calcium salt and soluble phosphate as the precursor of inorganic phase nano hydroxyapatite (nHAP),and the soluble salt and soluble ferrous salt as precursor to inorganic phase superparamagnetic nano Fe3 O4 particles were used to prepare Fe3 O4-CS-Col-nHAP composite scaffold by in situ fabrication and freeze-drying technique.The composition and morphology of Fe3 O4-CS-Col-nHAP composite scaffold and the distribution of nano particles were characterized by FTIR spectros-copy,XRD and SEM.And the magnetic energy of the scaffold was tested by physical property tester (PPMS).The results show that Fe3 O4-CS-Col-nHAP composite scaffold presents a homogeneously interconnected macroporous structure which has an everage size of around 100-150μm and the porosity is about 95%.And the low crystallinity of nHAP crystals and nano Fe3 O4 particles are uniformly distributed in the organic matrix.The nano Fe3 O4 particles prepared by in situ fabrication has super paramagnetic properties,with the increase of magnetic nano particles,the magnetic saturation strength is enhanced.The saturation magnetization intensionsity of Fe3 O4-CS-Col-nHAP com-posite scaffold is about 0.025 emu/g.Therefore,the hybrid nanostructure Fe3 O4-CS-Col-nHAP composite scaffold prepared by in situ fabrication and freeze-drying technique has good magnetocaloric effect,and is promising for bone repair application in bone tissue engineering.%为了制备具有磁热效应的多相杂化纳米复合材料,以可溶性钙盐和磷酸盐作为纳米羟基磷灰石(nHAP)的前驱体、可溶性铁盐和亚铁盐作为纳米Fe3 O4的前驱体,并结合壳聚糖(CS)和胶原(Col)两种有机基体的优越特性,通过原位复合和冷冻干燥技术,制备了纳米Fe3 O4-CS-Col-nHAP复合支架材料.通过FTIR、XRD、SEM、物理性能测试仪(PPMS)等方法对复合支架的组成、结构、形貌和磁性等方面进行表征.结果表明:纳米Fe3 O4-CS-Col-nHAP复合支架具有多级孔径结构,孔径尺寸约为100~150μm,孔隙率约为95%;低结晶度的nHAP晶体和纳米Fe3 O4颗粒均匀分布在有机基体上;通过原位复合技术制备的纳米Fe3 O4具有超顺磁性,随着磁性粒子含量的不断增加,磁饱和强度不断增强,饱和磁化强度为0.025 emu/g.通过原位复合和冷冻干燥技术制备的多相杂化的纳米Fe3 O4-CS-Col-nHAP复合材料具有良好的磁热效应,有望在骨修复组织工程中得到广泛应用.