Preparation and characterization of a bio-composites scaffold containing nano-hydroxyapatite/carboxymethyl chitosan

摘要

In this study, a genipin cross linked scaffold containing nano-hydroxyapatite (n-HAp) and carboxymehthyl chitosan (CMCS) was developed by freeze drying technique. The scaffolds were characterized using FTIR, XRD and SEM. The cytotoxicity of the scaffolds was compared with scaffolds cross linked by glutaraldehyde, and the Young's modulus was also tested. FTIR and XRD results indicated that CMCS's hydroxyl group, amino and amide regulated the n-HAp crystallization process, which results in the nano homogenous distribution of n-HAp and provided nano topographical features for nanohybrid scaffolds. SEM images revealed the scaffolds had porous structure and the pores were interconnected with an average diameter of 150 μm, which was profit for the growth of tissues. Cell morphology showed the genipin cross linked scaffolds had less toxicity and more facility for adhesion and proliferation of cells. Great mechanical properties of the scaffolds indicate their potential use in bone tissue engineering.