SERS 标记方法在生化分析中的应用

摘要

Surface-enhanced Raman scattering (SERS)label method is a novel bioassay technology,which combines the modern bio-label technology and SERS spectroscopy.In present method,the signal of the Raman report mole cules, acting as the label tracer signal,is enhanced by adsorbing on the noble metal (i.e.Ag and Au)nanoparticles.With the advantages of high sensitivity,high specificity,fast and easy detection in biochemical analysis,the study on SERS label method has been a hot topic.Here,SERS labeling method has been introduced firstly and its applications recently in the labeled immunoassay,tumor targeting,DNA molecular recognition and microbiological detection have been reviewed.At last,future prospect in this field are indicated.%表面增强拉曼散射（SERS）标记方法结合现代生物标记方法与 SERS 光谱技术，使吸附到金或银等贵金属表面的标记分子的拉曼信号显著增强，并将其作为标记示踪信号，具有生物兼容性好、灵敏度高、分子特征性强和快速简便等优点，已成为新颖的标记示踪技术的研究热点之一。本文综述近年来 SERS 标记技术应用于基因分析、蛋白质检测、微生物检测、肿瘤靶向和小分子物质的最新进展，着重介绍蛋白质和小分子物质的检测，并展望了今后的发展方向。