星状神经节电刺激建立交感神经介导的急性房颤犬模型

摘要

目的 建立交感神经张力异常介导的急性房颤动物模型的方法学.方法 将16只随意来源犬分为三组:对照组(n=4),右侧星状神经节(RSG)组(n=6)和左侧星状神经节(LSG)组(n=6),测定心房和肺静脉不同部位的房颤诱发率、房颤持续时间.结果 RSG刺激显著增加右心房(RA)的房颤诱发率和持续时间(P<0.05),LSG刺激显著增加左心房(LA)、左上肺静脉(LSPV)、左下肺静脉(LIPV)的房颤诱发率和持续时间(P<0.05);与刺激时相比,RSG切除显著降低RA的房颤诱发率和持续时间(P<0.05);LSG切除显著降低LA、LSPV、LIPV的房颤诱发率和持续时间(P<0.05).结论 星状神经节电刺激同时快速心房起搏6h可成功建立交感神经介导的急性房颤犬模型,星状神经节电刺激使心房和肺静脉部位的房颤诱发率显著升高,房颤持续时间显著延长,去星状神经节支配可减少房颤的发生和维持.%Objective To develop the methodology of establishing animal model of acute atrial fibrillation ( AF) induced by increased sympathetic nerve activity. Methods Sixteen mongrel dogs weighing 18 -25 kg were divided into 3 groups; Control group (n =4) underwent 6-hour rapid atrial pacing only; Right stellate ganglion (RSG) group (n =6) and left stellate ganglion (LSG) group (n =6). AF induction rate and AF duration in the left atrium (LA) , right atrium ( RA) , left superior pulmonary vein (LSPV) and left inferior pulmonary vein (LIPV) were measured. Results (1) The effect on AF inducibility: In the RSG group, the induction rate of AF was significantly increased in the RA ( P < 0. 05 ) compared with the baseline. However, there were no significant changes in the LA, LSPV and LIPV. In the LSG group, the induction rate of AF was significantly increased ( P < 0. 05 ) , compared with the baseline in the LA, LSPV and LIPV, respectively. Compared with RSG stimulation, right stellate ganglionectomy markedly decreased the AF induction rate of RA ( P < 0. 05 ) . Compared with LSG stimulation, left stellate ganglionectomy markly decreased the AF induction rate of LA, LSPV and LIPV (P < 0. 05). (2) The effect on AF duration : In the RSG group, the duration of AF was significantly prolonged in RA ( P < 0. 05 ) , compared with the baseline. In the LSG group , the duration of AF was significantly prolonged ( P < 0. 05 ) , compared with the baseline in the LA, LSPV and LIPV, respectively. Compared with RSG stimulation, right stellate ganglionectomy markedly shortened AF duration of RA (P < 0. 05 ) . Compared with LSG stimulation, left stellate ganglionectomy markedly shortened AF duration of the LA , LSPV and LIPV ( P < 0. 05 ) . Conclusions Electrical stimulation of unilateral stellate ganglion plus rapid atrial pacing for 6 hours can successfully establish a canine model of acute AF mediated by sympathetic nerve. Stellate ganglion stimulation promotes AF induction and prolong AF maintenance in atrial and pulmonary sites. The inhibition of sympathetic nerve activation by unilateral stellate ganglionectomy can reduce the AF initiating and sustaining.