具有拮抗关系的18个香灰菌株遗传差异性分析

摘要

Genetic diversity among 18 antagonistic strains of Xiang-hui-jun,the cohabitant fungus of Tremella fuciformis that plays a vital role in the growth and development of the mushroom,was analyzed using randomly amplified polymorphic DNA (RAPD),sequence-related amplified polymorphism(SRAP)and inter-simple sequence repeat (ISSR)markers.Eight RAPD primers generated 59 DNA bands from the 18 strains of which 49 (83%)were polymorphic,seven SRAP primers generated 49 DNA bands of which 47 (96%)were polymorphic,while six ISSR primers generated 60 DNA bands of which 50 (83%)were polymorphic.A dendrogram based on RAPD、ISSR and RAPD analyses was constructed using the unweighted pair-group method with arithmetic means,and the 18 strains were delineated into seven groups at a dissimilarity coefficient level of 0.48 as follows:strains Hp.sp0006,TR Boxi,Wakeng wild,TR Fengshu and TR8801 Fengshu each constituted individual groups (Groups 1-5),Group 6 comprised strains Hp.sp0052 and 69,and the remaining 11 strains (Hp.sp0004,Hp.sp0010,T0050,T0048,T0036,TR23,TR5,TR95,71,TR03 and TR86)clustered together to form Group 7.%香灰菌(xiang-hui-jun)是银耳(Tremella fuciformis)的伴生菌,对银耳的生长、发育具有至关重要的作用。采用 RAPD、SRAP 和 ISSR 三种分子标记对具有拮抗关系的18个香灰菌菌株进行遗传差异性分析。结果表明：8对 RAPD 引物共扩增出59个位点片段,多态性条带总共为49条,占总片段条数的比率为83%；7对 SRAP 引物共扩增出49个位点片段,多态性条带总共为47条,占总片段条数的比率为96%；6对 ISSR 引物共扩增出60个位点片段,多态性条带总共为50条,占总片段条数的比率为83%。聚类分析表明,当以相异系数0.48为阈值时,可将这些香灰菌株分成7个类群：Hp.sp0006、TR 柏溪、瓦坑野生、TR 枫树和 TR8801枫树分别自成一个类群,Hp.sp0052和69为一个类群,其余11个(Hp.sp0004、Hp.sp0010、T0050、T0048、T0036、TR23、TR5、TR95、71、TR03和 TR86)菌株归为一个类群。