目的:考察AB-8大孔吸附树脂对藏茵陈黄酮提取物的吸附分离性能,并探讨AB-8树脂的纯化工艺条件.方法:采用静态吸附分离法确定AB-8大孔吸附树脂的吸附性能及分离条件;以总黄酮吸附量、总黄酮回收率为考察指标,采用紫外分光光度法测定总黄酮.结果:AB-8大孔吸附树脂对藏茵陈总黄酮的回收率为89%,其分离藏茵陈总黄酮的工艺条件为:藏茵陈总黄酮上样质量浓度为50mg·mL-1,藏茵陈总黄酮最大吸附量为32.43mg·mL-1,吸附体积流量为2mL·min-1,洗脱剂为50%乙醇,洗脱剂用量为7倍柱体积,树脂可重复使用3次.结论:采用AB-8大孔吸附树脂吸附分离藏茵陈总黄酮的性能最佳,值得推广应用.%Objective: To set up the technology for adsorbing and separating total flavone in Zangyinchen with macroporous adsorption resin, and AB -8 macroporous adsorption resin was invested. Methods: The static adsorption and separation were used in investigation of macroporous adsorption and separation; the dynamic adsorption and adsorption were used in studying the condition of adsorption and separation. The total flavone adsorption capacity and total flavone recovery rate were used as the evaluation criteria. The UV spectrophotometric method was used in the determination of total flavone content. Results: The results showed that the AB -8 was the best for adsorbing and separating the total flavone in Zangyinchen in the following technological condition: the concentration of Zangyinchen sample extract was 50mg/mL;the maximum adsorbing capacity for total flavone in Zangyinchen was 32.43mg/mL; the current velocity was 2 mL/min;the eluting reagent was 50％ ethanol;and the AB -8 resin could be used three times repeatedly. Conclusion: It is the best way to use AB -8 resin to separate the total flavone in Zangyinchen under the technological conditions, which is worth promoting and using.
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