目的:建立RP-HPLC方法,同时测定复方脂质体中阿霉素(DOX)与槲皮素(QUE)的含量.方法:Kromasil C18柱(250mm ×4.6mm,5μm),甲醇-乙腈-pH 3.8磷酸缓冲盐(19∶ 29∶ 52)等度洗脱,流速1 mL/min,检测波长254nm,柱温25℃.结果:DOX与QUE分别在0.50 ～ 10.00μg/mL、0.03～2.00μg/mL范围内线性良好(r分别为0.999 7、0.999 4)；低、中、高浓度回收率分别为(99.53±2.56)％、(103.12±0.37)％、(103.21±2.02)％和(96.10±1.38)％、(102.30±1.76)％、(103.78±1.06)％(n=3).结论:本方法准确,重复性好,可用于同时测定阿霉素-槲皮素复方脂质体中两种药物的含量.%Objective: To establish a method for the simultaneous determination of doxorubicin and quercetin in Doxorubicin - Quercetin Compound Liposomes by RP - HPLC. Methods: The contents of doxorubicin and quercetin in Doxorubicin - Quercetin Compound Liposomes were determined by RP - HPLC. The determination was performed on Kromasil C18(250mm × 6mm,5μm) column with mobile phase composed of methanol - acetonitrile - PBS( pH 3. 8) (19:29:52) at the flow rate of 1. 0mL ·min-1. The detection wavelength was set at 254 nm, column temperature was 25℃ and injection volume was 30℃ Results: The linear ranges of DOX and QUE were 0. 5 ～ 1. 0μg·mL-1 (r = 0. 999 7) and 0. 03 ～ 2. 0μg·mL-1 (r = 0. 999 4) , and the average recoveries of DOX and QUE in three concentration levels were (99. 53 ±2.56)% ,(103. 12 ±0. 37)% ,(103. 21 ±2. 02)% and(96. 10 ±1. 38)% ,(102. 30 ±1. 76)% , (103. 78±1.06)%(n = 3). Conclusion; The method is accurate, rapid and reproducible for the concentration determination of doxorubicin and quercetin in Compound Liposome at the same time.
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