Under microwave assisted heating, uridine imprinted polymer was prepared rapidly using uridine as template, acrylamide (AA) as monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker. The results indicated that the polymerization time was shorten significantly, only one eight of the conventional heating. Infrared spectroscopy and scanning electron microscopy were used to characterize the structure and morphology of the imprinted polymer, and the results showed that the imprinted polymer was coated on the surface of silica microspheres successfully. Well-dispersed particles are observed and the thickness of the imprinted shell is about 100 nm. Combined with high liquid chromatography technique, on-line imprinted enrichment and determination of nucleotide ingredients from the extracting solution of isafidis root were performed successfully.%采用微波辅助加热,以尿苷为模板分子,丙烯酰胺(AA)为功能单体,乙二醇二甲基丙烯酸酯(EGDMA)为交联剂,快速制备尿苷印迹聚合物,聚合时间大大缩短,仅为传统加热的1/8.采用红外光谱和扫描电镜对印迹聚合物进行表征,结果表明二氧化硅表面成功包覆尿苷印迹壳层;该印迹聚合物颗粒分散均匀,印迹壳层厚度约为100 nm.将该印迹聚合物填充萃取柱用于核苷类组分在线富集,结合高效液相色谱仪技术,实现了板蓝根提取液中尿苷、鸟苷和腺苷在线富集和检测.
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