光皮桦组织培养离体再生研究

摘要

采用正交试验等设计,系统开展了光皮桦组织培养高效再生体系研究.结果表明:光皮桦茎段外植体最佳诱导培养基及激素组合为MS+0.50 mg·L-1 6-BA+0.10 mg·L-1 TDZ+30 mg·L-1蔗糖+5.50 g·L-1琼脂,丛生芽最佳生根培养基为1/2MS+1 mg·L-1 IBA+20 g·L-1蔗糖+5.50 g· L-1琼脂；丛生芽在最佳生根培养基上培养15d后获健壮生根苗,移栽成活率达90％.该实验结果为光皮桦的优良品种快繁以及遗传转化体系建立奠定了良好的基础.%With orthogonal experiment design,a systematic research of tissue culture and efficient regeneration system of Betula luminifer was carried out. The appropriate medium for differentiation of stem ex-plants was MS+0. 50 tng · L-1 6-BA + 0. 10 mg · L-1 TDZ+30 g · L-1 sugar+5. 50 g · L-1 agar,and the suitable rooting medium was 1/2MS+1 mg · L-1 IBA + 20 g · L-1 sugar+5. 50 g · L-1 agar. The survival rate reached to 90%. These results could lay a good foundation for multipropagation and genetic transformation of B. Luminifera.