Multiple alignment of 22 NAC transcription factors, including 14 abiotic stress related NAC transcription factors and 8 NAC transcription factors involved different biological functions ,was done and phy-logenetic tree was built. Analysis and prediction of the amino acid composition,physical and chemical properties, hydrophilicity/hydrophobicity, conservative domain, the secondary structure, sub-cellular localization, and tertiary structure of 14 abiotic stress related NAC transcription factors were done with the help of bioinformatics tools. The results showed that 22 NAC transcription factors were divided into two groups, including a group of abiotic stress related NAC transcription factors and the rest ones; A,B,C,D and E subdomains constituted the NAC domain which were in N-terminus of 14 abiotic stress related NAC transcription factors, 3 common motifs were also located in NAC domain; The C-terminus contained several conservative ammo acid residues and many phosphorylation sites, was involved in transcriptional regula-tion;A,C,D suSdomains were the main hydrophilic areas of abiotic stress related NAC proteins,and most of them were tocated in nucleus ,only a few ones in cytoplasm or mitochodria;crhelix and (3-sheet were the primary secondary structure of abiotic stress related NAC proteins;Similarity of tertiary structure of RD26 and ANAC indicated that they would have the similar functions.%通过生物信息学手段,对22种NAC蛋白(14种非生物逆境胁迫相关NAC蛋白、8种涉及不同生物学功能的NAC蛋白)进行氨基酸序列比对和系统发生树构建,对14种非生物逆境胁迫相关NAC蛋白氨基酸组成、理化性质、亲/疏水性、保守结构域、亚细胞定位、二级结构及三级结构等进行了分析、预测.结果显示,22个NAC蛋白中,非生物逆境相关的NAC蛋白聚成一类,其余NAC蛋白聚为另一类；非生物逆境胁迫相关的NAC蛋白序列分析显示,N-端具有A、B、C、D、E5个保守的亚结构域,共同组成NAC结构域,C-端含有多个保守的酸性氨基酸位点,具有转录激活功能,同时蛋白中含有多个丝氨酸(S)、苏氨酸(T)和酪氨酸(Y)磷酸化位点；非生物逆境胁迫相关NAC蛋白主要亲水区域位于A、C、D亚域,大多定位于细胞核,个别定位于细胞质或线粒体,二级结构则以α-螺旋和β-折叠片为主；拟南芥RD26和ANAC三级结构上的一致性暗示了功能上的相似.
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