不同LEDs复合光质对秋葵组培苗生长和生理特性的影响

摘要

以秋葵品种‘清福’为试材,采用单因素随机区组设计试验,将秋葵顶芽作为外植体(1.0 cm)接种到添加了0.05 g· L-1NAA的MS基本培养基上,然后分别转入荧光灯(CK,对照)、蓝红复合光(BR3∶1、BR1∶1、BR1∶3、BR1.4)、蓝红黄(BRY=1∶3∶1)、蓝红绿(BRG=1∶3∶1)和蓝红远红复合光(BRFR=1∶3∶1)下进行光照射,考察不同光质对秋葵组培苗生长和生理特征的光效应,筛选适合秋葵组培苗的最优光质.结果显示:(1)不同复合光质处理的秋葵组培苗鲜质量、干质量、根长、茎长和茎粗不同程度增加,并均以BRY处理最高.(2)不同复合光质处理的组培苗根系活力都显著高于对照,且以BRY处理下最大;各光质处理下的幼苗叶片叶绿素和类胡萝卜素含量也以BRY处理最高,并显著高于其余处理.(3)组培苗叶片中SOD、POD和CAT的活性均以BRY处理下最强,CK处理下最弱.(4)各处理组培苗叶片中的可溶性糖、蔗糖、可溶性总碳含量和淀粉含量均以BRY处理最高,其次为BRG处理,CK下较低;叶片可溶性蛋白、游离氨基酸和可溶性总氮的含量均以BRY处理最高,其次为BRG处理,CK下最低.研究表明,蓝红黄复合光显著提高了秋葵组培苗根系活力、光合色素含量、抗氧化酶活性及碳氮代谢和光合产物累积,从而有效促进了秋葵组培苗的生长,可以作为秋葵离体培养的优选光质.%Shoot apical bud cuttings of okra cultivar ‘Qingfu'in vitro (1.0 cm) were transplanted on MS basal medium supplemented with 0.05 g · L 1 naphthalene acetic acid (NAA) and cultured under eight different quality lights:fluorescent lamp (CK),four mixture blue plus red light emitting diodes (BR3∶ 1,BR1:1,BR1:a,BR1:4 LEDs),blue,red plus yellow (BRY=1 ∶ 3 ∶ 1) LEDs,blue,red plus green (BRG=1 ∶ 3 ∶ 1) LEDs,blue,red plus far-red (BRFR=1 ∶ 3 ∶ 1) LEDs.The objective of present study was to determine the effects of different LEDs lights on the growth and physiological characteristics of okra (Abelmoschus esculentus) plantlets,and screen for optimal quality of okra plantlets in vitro in order to provide theoretical guidance and technical support of okra rapid propagation in vitro.The results showed that.(1) fresh mass,dry mass,root length,stem length and stem width were the greatest in plantlets cultured under BRY LEDs light;(2) root activity showed significantly higher in plantlets cultured under different lights than that of CK,and the highest was under BRY LEDs;Photosynthetic pigments were the highest in plantlets cultured under BRY LEDs,and which were also higher than that of other treatments;(3) the activities of SOD,POD and CAT were the greatest in plantlets cultured under BRY LEDs,and the lowest was that of CK;(4) the concentrations of soluble sugar,sucrose,soluble carbon and starch were the greatest in seedlings under BRY LEDs treatment,followed by BRG,and the lowest under CK;The concentrations of soluble protein,free amino acid and soluble nitrogen were the greatest in seedlings under BRY treatment,followed by BRG,and the lowest under CK.The present results indicated that larger and healthy plantlets and greater biomass of okra were produced in the presence of BRY (1 ∶ 3 ∶ 1) LEDs light.BRY light significantly promoted the root activity,photosythetic pigment content,antioxidant enzyme activities,carbon and nitrogen metabolism and accumulation of photosynthetic product of okra plantlets,which was the preferred light quality for the okra in vitro culture system.