中间锦鸡儿WRKY75基因对拟南芥耐受盐和ABA能力的影响

摘要

该研究从旱生灌木中间锦鸡儿中克隆得到1个CiWRKY75基因.序列分析显示,CiWRKY75开放阅读框长570 bp,编码189个氨基酸,含有1个WRKYGQK 基序和1个C2H2型锌指结构,属于第二类WRKY转录因子.亚细胞定位显示,CiWRKY75定位于细胞核.实时荧光定量 PCR检测表明,CiWRKY75基因的表达受盐胁迫和ABA诱导.在拟南芥中过量表达 CiWRKY75后,与野生型拟南芥相比,转基因株系种子的萌发率在盐胁迫下降低,并且对盐胁迫的耐受能力明显减弱;ABA处理下,2个转基因株系的种子萌发率(10.3%、9.6%)较野生型(25.9%)明显降低.研究表明,CiWRKY75是中间锦鸡儿对盐和ABA响应的重要调控因子.%In this study,we cloned a WRKY transcription factor encoding gene WRKY75 from Caragana intermedia.The sequence analysis shows that the open reading frame of CiWRKY75 is 570 bp and encodes 189 amino acids,contains one WRKYGQK motif and a C2H2-type zinc finger,belongs to group II WRKY family.CiWRKY75 localized in the nuclei by transforming the GFP fusion constructs into A rabidopsis thaliana mesophyll protoplast.The transcripts of CiWRKY75 was induced by NaCl and ABA treatments. Overexpression of CiWRKY75 in Arabidopsis thaliana resulted in sensitivity to salt tolerance,and the germination rate of the transgenic lines decreased under both NaCl and ABA treatments compared with wild type.These results indicates that CiWRKY75 is an important regulator in plants response to salt and ABA treatments.