利用BSR-Seq定位小麦品种郑麦103抗条锈病基因YrZM103

摘要

Zhengmai 103 is a new wheat cultivar highly resistant to stripe rust. In order to detect the stripe rust resistance gene in Zhengmai 103, a segregating population was developed by making cross between Zhengmai 103 and a highly stripe rust suscepti-ble wheat cultivar Nongda 399. The 214 F2derived F2:3 progenies were inoculated with mixed prevailing Pst races CYR32, CYR33 and CYR34 (V26) in field condition for disease resistance evaluation at adult plant-stage. Genetic analysis revealed that the stripe rust resistance of Zhengmai 103 is controlled by a single gene,temporarily designated YrZM103.By applying RNA-seq with bulked segregant analysis(BSA),six polymorphic markers were developed to map YrZM103 on chromosome 7BL flanked by markers ZM215 and ZM221 with genetic distances of 6.9 cM and 11.8 cM,respectively.Comparative genetic mapping indi-cated YrZM103 was located on different genetic interval from that of known stripe rust resistance genes on 7BL.%郑麦103是一个高抗条锈病的小麦新品种, 为明确其携带的抗病基因, 用郑麦103与感条锈病品种农大399杂交构建分离群体, 用条锈菌CYR32、CYR33和CRY34 (V26)混合菌系进行田间接种和成株期抗性鉴定, 对214个F2:3家系的条锈病抗性进行遗传分析,初步确定郑麦103 的抗条锈性由单个主效基因控制,定名为 YrZM103.通过BSR-Seq 技术开发了 6 个与 YrZM103 紧密连锁的分子标记,将 YrZM103 定位于染色体臂 7BL 分子标记 ZM215 和ZM221之间,遗传距离分别为11.8 cM和6.9 cM.利用7BL染色体上与其他已知抗条锈病基因紧密连锁的分子标记进行比较作图,发现YrZM103是不同于7BL末端其他抗条锈病基因的新基因.