四倍体马铃薯熟性连锁SCAR标记的开发与验证

摘要

Maturity is one of the most important quantitative traits in potato. In this study, a maturity linked molecular marker SCAR5-8 was developed using Zhongshu 3, Zhongshu 19 and their F1 segregation population consisting of 221 individuals via reduced-representation sequencing and bulked segregant analysis. A further testing of marker SCAR5-8 was performed using 53 early maturity individuals, 63 late maturity individuals and 70 tetraploid varieties, showing the selection accuracy 87.1% and 81.4% between marker test and phenotyping in segregation population and tetraploid varieties, respectively. Moreover, Pearson's correlation analysis also showed that the marker SCAR5-8 was significantly correlated to maturity trait in potato. The developed SCAR5-8 marker could be used in marker-assisted selection.%熟性是马铃薯的重要数量性状之一.本研究以马铃薯早熟品种中薯3号和晚熟品种中薯19及其熟性分离群体(221份)为材料,高通量简化基因组测序和集群分离分析(BSA)相结合,开发获得一个SCAR标记,命名为SCAR5-8.进一步利用该标记对分离群体的53份早熟和63份晚熟子代及70份四倍体马铃薯品种进行验证,结果表明,该标记在分离群体和四倍体品种中的检测结果与表型鉴定结果总体吻合度分别达87.1%与81.4%,Pearson's双侧相关分析显示,其相关性均达极显著水平.该SCAR标记可被用于标记辅助选择,对加速四倍体马铃薯育种进程具有重要意义.