四倍体马铃薯块茎蛋白含量分子标记的开发与验证

摘要

块茎蛋白含量是马铃薯的重要品质性状之一, 相关分子标记的开发研究较少.本研究以马铃薯高蛋白品种大西洋、低蛋白品种定薯1号及包含173份子代的蛋白含量分离群体为材料, 通过高通量简化基因组测序技术与集群分离分析(BSA)相结合, 开发了 SCAR8-107 标记, 并以此标记对分离群体的 74 份高蛋白、42 份低蛋白子代及 54 个四倍体马铃薯品种验证表明, SCAR8-107 标记在后代分离群体和四倍体马铃薯品种中的检测结果与表型蛋白含量的对应度分别达到了90.51%和72.97%, 经过Pearson’s双侧相关性分析, 相关性均达到了极显著水平.SCAR8-107的开发对于四倍体马铃薯块茎蛋白含量标记辅助选择与加速马铃薯品质育种具有重要意义.%Protein content is one of the most important quality traits in potato. In this study, SCAR8-107, a protein content linked molecular marker, was developed using Atlantic, Dingshu 1, and their F1segregation population consisting of 173 individuals by reduced-representation sequencing and bulked segregant analysis. A further testing of marker SCAR8-107 was performed using 74 high protein content individuals, 42 low protein content individuals and some tetraploid varieties, showing the selection accu-racy of 90.51% and 72.97% between marker test and phenotyping in segregation population and tetraploid varieties respectively. Pearson’s correlation analysis also showed that the marker SCAR8-107 was significantly correlated to protein content in potato. The SCAR8-107 marker could be used in marker-assisted selection.