水稻抗白叶枯病相关基因的亚细胞定位

摘要

利用亚细胞定位技术对水稻抗白叶枯病相关基因的表达产物进行了分析，为基因功能分析提供了重要信息。通过前期基因芯片筛选、半定量/定量PCR的验证及基因功能注释，从高抗白叶枯病水稻品种Y73中筛选了18个潜在的与抗白叶枯病相关的基因，并在烟草叶片细胞中进行亚细胞定位分析。通过观察融合有绿色荧光标记（ sGFP）的表达产物，初步了解这些基因在烟草细胞中的表达位置。经过激光共聚焦显微镜观察后发现：其中4个基因的 sGFP 融合蛋白定位于细胞核上，可能发挥了转录因子的功能；4个基因的 sGFP 融合蛋白定位于细胞质膜上，推测可能与细胞质膜的稳定或控制蛋白转运相关；4个基因的 sGFP 融合蛋白同时定位于细胞核和细胞质膜上；另有6个基因的 sGFP 融合表达后没有观察到明显的定位信号。%The subcellular localization of rice bacterial blight related genes were examined to provide important information for the study of gene function .Eighteen candidate genes which potentially associated with bacterial blight resistance were selected from Y73 by using gene chip, semi-quantitative/quantitative PCR and function annotation . These candidate genes were fused with green fluorescent marker ( sGFP) and subcellular localization of their products in tobacco leaf cells .Four genes combined with sGFP were found mainly located in the cell nucleus , showing the typical characteristics of transcription factors; Four genes were found mainly located in the plasma membrane , indicating their potential functions of maintaining the stability of the cell membrane or in transport control on cell membrane;Four genes were simultaneously located in both cell nucleus and the plasma membrane and there was no apparent subcellular localization signal for the remaining 6 genes combined with sGFP .