莲藕腐败病病原菌生物学特性研究

摘要

为明确莲藕腐败病病原菌的生物学特性，研究了不同培养基及光照、温度、pH、碳氮源对莲藕腐败病病原菌菌丝生长的影响。结果表明，该菌菌丝在4种培养基上均能较好生长。光照对病原菌的生长无显著影响。在20～30℃范围生长较快，最适温度为28℃。病原菌在pH值4～13范围内均能生长，最佳pH值为7。不同的碳源中，麦芽糖、蔗糖、果糖较适合该菌的生长；在供试的氮源中，菌丝在含酵母浸出粉的基础培养基上生长最快。%The effects of culture medium, photoperiod, temperature, pH degree, carbon and nitrogen source on the biological characteristics of Fusarium oxysporum Schl.f.sp.Nelumbicola ( Nis.&Wat.) Booth, pathogen of Rhi-zome rot of lotus root Nelumbo nucifera Gaertn, were studied.The results showed that the mycelia could grow well on four tested culture mediums under 20~30℃, and the optimum temperature was 28℃.Different photoperiods had no significant influences on the growth of mycelium.It was found that the range of pH was 4~13 and the optimum was 7.The optimal carbon source was maltose, sucrose and fructose, and the optimal nitrogen source was yeast extract powder.