中国荷斯坦牛LF基因多态性与泌乳性状及乳腺炎的关联分析

摘要

Astract:Lactoferrin(LF) is one of the defense factor in breast tissue , which plays an important role in body's resist-ance to mastitis.The polymorphism of LF gene in 303 Holstein cattle in Ningxia was detected by PCR-SSCP, and the effects of LF gene g.4388G>C locus mutation on daily milk yield , milk fat, milk protein percentage and somatic cell score ( SCS) were analyzed via general linear model .It was shown that there was a significant relationship be-tween LF gene g.4388 G>C locus mutation and milk yield, milk fat and SCS (P<0.05).The GC genotype had significantly lower SCS compared with GG and CC genotypes (P<0.01).Therefore, GC gene as a superior genotype against mastitis would be applied in dairy cattle breeding .%乳铁蛋白（Lactoferrin，LF）是保护乳腺组织的防御因子之一，在机体抵抗乳腺炎方面起着重要作用。本研究采用PCR-SSCP技术和测序的方法对宁夏农垦303头中国荷斯坦牛的LF基因进行了遗传多态性分析，利用一般线性模型分析LF基因g．4388 G＞C位点突变对日产奶量、测定日乳脂率、测定日乳蛋白率及体细胞评分4个泌乳性状的影响。结果显示，LF基因g．4388G＞C突变位点与日产奶量、乳脂率和体细胞评分值存在显著关联（P＜0.05）。多重比较结果表明，GC基因型个体的体细胞评分（SCS）值极显著低于GG和CC基因型个体（ P＜0.01），因此，可尝试将GC基因型作为乳腺炎抗性的优良基因型应用于奶牛育种中。