紫红薯多糖的提取纯化及抗氧化作用研究

摘要

The technology for extraction of polysaccharide from purple sweet potato was optimized by orthogonal array design. After removal of pigment and protein by X - 5 and sevag method respectively, Ⅰ and Ⅱ of polysaccharide portions were obtained by further purification by cellulose Chromatography DEAE. Addition ally , the antioxidant capacity of the purified polysaccharide was evaluated by determining their DPPH ? And ? OH and ABTS scavenging activities. The results indicated that the obtained optimum conditions are material to wa ter ratio 1:8, extraction time 60 min, extraction power 240 W. The yield of polysaccharide is 5.44% under the optimal conditions. After purification;Ⅰ and Ⅱ of polysaccharide portions showed considerable antioxidant capacity.%实验优化了从紫红薯中提取多糖的工艺,利用DEAE - 52柱层析分离纯化出紫红薯多糖Ⅰ和Ⅱ两个组分,并测定了对·OH、DPPH·和ABTS的清除作用.结果表明:紫红薯多糖最佳提取条件为提取时间60 min、料液比为1:8、超声功率为240W,经纯化后的紫红薯多糖I和Ⅱ具有较好的清除自由基的能力.