表面活性剂协同微波提取大豆荚黄酮的研究

摘要

Surfactant and microwave extraction was applied to the extraction of flavonoids from soybean pods.Response surface methodology (RSM) was applied to optimizing the extraction conditions.Based on the signal factor test, microwave power, the ratio of liquid to solid and dodecyl dimethyl betaine (BS- 12) mass ratio, were chosen as casual factors, and a three - factor and three - level Box - Behnken central composite design was performed to attain the highest weighed value of yields of soybean pods flavonoids.RSM method was used to investigate the three factors impacting the response value of yields of soybean pod flavonoids.The optimal microwave extraction assisted with surfactant conditions was determined as follows: extraction time 4 min 20 s, ratio of liquid to solid 26 (mL: g), dodecyl dimethyl betaine 0.44％ and microwave power 400 W.Under these conditions, the extraction ratio of soybean pod flavonoids was 9.36％, which was consistent with the predicted values.These results demonstrate the validity of microwave extraction assisted with surfactant process for flavornoids in soybean pods.%研究表面活性剂协同微波辐射提取大豆豆荚中总黄酮的工艺条件.在单因素试验的基础上,选择微波提取时间、液料比和十二烷基二甲基甜菜碱(BS-12)含量为随机因素进行三因素三水平的Box-Behnken中心组合设计,采用响应面法(RSM)分析3个因素对响应值的影响.结果表明,提取大豆豆荚黄酮的最佳工艺条件为:BS-12用量为4.4 g/L、微波提取时间4 min 20 s、液料比为26 (mL:g)、微波功率为400 W.在此条件下,大豆豆荚黄酮提取率达到9.36%,与理论预测值基本吻合,说明响应面法优化表面活性剂协同微波提取大豆豆荚总黄酮可行.