利用重组PCR技术构建大肠杆菌肠毒素LTB-STI融合基因

摘要

利用重组PCR技术将从原始菌种中扩增得到的LTB、STI基因片段连接起来，构建了LTB-STI融合基因。并将其克隆到pUCm-T载体上，转化到大肠杆菌DH5α中，得到克隆T-LS，序列分析表明该克隆具有正确的基因序列和阅读框，具有起始密码子ATG和终止密码子TAA，可以插入到原核表达载体中进行表达。%The 5' terminus of STI gene was fused to the 3' terminus of LTBgene by recombinant PCR. Then the LTB-STI fusion gene was cloned into pUCm-T vector, transformed into E.coli DH5α, and sequenced using universal primers. The gene sequence determination showed the correct sequence and reading frame.