P22 - PMAP36 ETEC The composition of the enterotoxigenic Escherichia coli ghost vaccine candidate by recombinant P22 lysozyme-PMAP36 fusion protein against neonatal piglet colibacillosis

摘要

The present invention relates to an enterotoxigenic excherichia coil (ETEC) ghost vaccine composition using a recombinant p22 lysozyme-PMAP36 fusion protein for preventing colibacillosis of a weaning pig. ETEC strains expressing F4 and F18 fimbria are induced to be ghost by a p22 lysozyme-PMAP36 fusion protein, and an effect of induced cells as an ETEC ghost vaccine is evaluated. In order to evaluate the efficacy of a new preventive vaccine candidate material, the preventive vaccine candidate material is intramuscularly inoculated to a weaning piglet, and the resistance to colibacillosis after weaning is confirmed. In other words, each fimbria-containing ghost vaccine is intramuscularly inoculated to the piglet of a group B at 2 weeks after birth to have 2×10^9 cells, and a PBS is intramuscularly inoculated to weaning piglet of a control group A. The titer of serum IgG of the piglet of the group B is significantly increased compared to that of an antibody of the piglet of the group A. After, the piglet is infected by a wild-type ETET, the diarrhea and mortality are not observed in all piglets of the group B but a diarrhea symptom is observed in 70% of the piglets of the group A, and 30% of a mortality rate is shown therein. Accordingly, enterotoxigenic colibacillosis after weaning is confirmed to be effectively resisted when a newly prepared vaccine is intramuscularly inoculated to the weaning piglet.