青稞慢性消化淀粉制备条件优化的研究

摘要

Hordeum vulgare var.nudum starch was used as a raw material,slowly digestible starch (SDS) was prepared respectively by pullulanase,α-amylase and β-amylase methods,and the SDS-preparing conditions were optimized.The orthogonal experiments showed that the pullulanase method of preparing SDS was the best, and the gained SDS content was 32.72%.The pullulanase method’s treatment conditions were that the added pullulanase amount was 200 U,the enzymolysis time was 10 h,the refrigerated resuscitation time was 1 d,and the starch concentration was 15%.%以青稞淀粉为原料，分别以普鲁兰酶法、α-淀粉酶法、β-淀粉酶法制备慢消化淀粉（Slow digestion of starch，SDS），并优化 SDS 的制备条件。通过正交实验确定制备 SDS 的最佳方案为普鲁兰酶法，制备得到的 SDS含量为32．72％。普鲁兰酶法处理条件为：普鲁兰酶的添加量200 U，酶解时间10 h，冷藏回生时间1 d，淀粉浓度15％。