芦笋游离小孢子培养研究初报

摘要

In this paper, the isolated microspore culture is studied with five cultivars or hybrid combinations of Asparagus officinalis. The results show that the optimal time for the inducement of callus is near the monokaryotic stage; there is significant difference in the inducement rate of callus among different genotypes; the frequency of the inflated microspore treated by different temperatures is 6 times higher than that of the control, and the highest frequency reaches 22.5％; the suitable medium for the isolated microspore culture is MS + 0.5 mg/L 6 - BA + 1.0 mg/L NAA + 0.2 mg/L 2,4 - D + 400 mg/L Glu + 6％ sucrose.%以5个芦笋品种不同发育时期的花药为试验材料,进行芦笋游离小孢子培养,探索高频率获得雄核发育个体的技术途径,为芦笋全雄育种和杂种优势利用服务.初步试验结果表明:单核靠边期是小孢子适宜培养的最佳时期:不同的基因型之间小孢子愈伤组织诱导率差异显著;对花药小孢子进行变温预处理,其膨大率比对照高出6倍多,最高达到22.5%;小孢子培养的适宜培养基为Ms加0.5 mg/L 6-BA、1.0 mg/L NAA、0.2 mg/L 2,4-D.400 ms/L Glu和6%的蔗糖.