乙醇发酵分离耦合过程中负压对酵母代谢的影响

摘要

The effects of negative pressure on yeast reproductive capacity, yeast morphology, fermentation performance, fermentation byproduct in the coupling process of ethanol fermentation and separation were studied. The results showed that negative pressure had a certain influence on the reproductive capacity and morphology of yeast by plate and microscopic observation, and the maximum ethanol concentration and ethanol productivity of the samples in negative pressure treatment were higher than those in the control. During pumping the air, a part of sugar was transformed into cell maintenance energy, which was proved by metabolic flux analysis. According to the results of fermentation experiment, the yeast fermentation kinetic model was established.%研究了负压对酵母繁殖力、酵母形态、发酵性能、发酵副产物等的影响,结果表明:通过平板和显微镜观察,发现负压对酵母的繁殖力和形态有一定的影响；抽样的最大乙醇浓度、乙醇生产力比对照高；抽压过程中有一部分糖被消耗转化为茵体的维持能；并根据发酵结果,对菌体生长、糖消耗、CO2生成、乙醇生成进行了曲线拟合.