烟草梗丝中含有大量果胶、纤维素等细胞壁物质，造成吸味不好，对其在烟制品中的应用造成了阻碍。本研究旨在利用GYC 501的发酵酶液处理烟草梗丝，以降解其中以果胶为主的多糖，改善吸味。本研究首先建立了一种测定烟草梗丝中果胶含量相对简易的方法，并从发酵时间、缓冲液浓度、缓冲液pH值、发酵温度、酶的使用量入手，先进行单因素优化实验再进行正交实验，以优化发酵条件。结果显示：使用塔宾曲霉（ Aspegr illus tubingensis） GYC 501在烟草梗丝培养基中发酵48 h后所得到的发酵液对烟草梗丝进行发酵处理，得到最优发酵条件：酶使用量为1536 U，发酵时间为12 h，温度为42℃，缓冲液pH为4．8，缓冲液浓度为0．3 mol／L，梗丝含水量为50％。在此最优发酵条件下，梗丝果胶降解率最佳，为34．97％。%There are a large amount of cell wall substances such as pectin and cellulose in tobacco stem , and these sub-stances cause bad smoking quality , thus tobacco stem application is limited in manufacture .This research treated tobacco cut-stem with the fermentation liquid of Asperig llus tubingenis s GYC 501 to degrade the polysaccharides ( mainly being pectin ) in it and to improve its smoking quality.A relative simple method for measuring the pectin content in tobacco cut-stem was built.The fermentation conditions ( fermentation time, buffer solution concentration, buffer solution pH-value, fermentation temperature, pectinase application rate, and so on) of tobacco cut-stem were optimized by single-factor experiment and orthogonal test.The fermentation liquid of A.t ubingensis GYC 501, which was obtained by culturing this fungus in the tobacco cut-stem liquid medium for 48 h, was applied to ferment the tobacco cut-stem.The optimal fermentation conditions were obtained as follows: pectinase application rate 1536 U, fermentation time 12 h, fermentation temperature 42℃, buffer solution pH 4.8, 0.3 mol/L buffer solu-tion, cut-stem moisture content 50%.Under the above optimum fermentation conditions , the degrading rate of pectin in tobacco cut-stem was the highest, reaching 34.97%.
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