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两种微生态制剂对草鱼消化相关酶活性的影响

     

摘要

选用以枯草芽孢杆菌为主要成分的Ⅰ制剂及混合菌的Ⅱ制剂两种常用、商品化微生物制剂,对辽宁省池塘养殖草鱼进行投喂、泼洒和混合使用试验,并比较测定鱼肝脏、肠道组织中淀粉酶、蛋白酶及脂肪酶活性值.结果表明,以调节水质为主要作用的泼洒制剂Ⅱ对草鱼体内消化相关酶活性影响不大,但以促进消化生长为主要作用的投喂制剂Ⅰ对酶活性有较大促进作用,而制剂Ⅰ/Ⅱ混用在调节水质、帮助消化的同时能最大程度的促进草鱼体内消化相关酶活性提升,有助于促进草鱼生长及繁殖发育.因此,微生态制剂在草鱼池塘养殖中,对鱼快速健康生长有较好的促进作用.%Two kinds of microecologics (named M- I and M-II ) ,which were based on the Bacillus subtilis and had already been developed and applied in the practice, were selected to determine their influence on several digestive enzymes in grass carp. M- I was used to mix with the feeding and M-E was poured into the water of the ponds. Four groups were set in this study,including the control group,M- I group,M-II group,and the combination group of these two microecologics. After the usage of microecologics for 3 months, the amylase, protease and lipase of the livers and intestines were determined and compared with each other. The results indicated that M-II prepara tions , which mainly functioned on regulating the water quality, did not affect the three digestive enzymes in grass carp, while M-I preparations,which could promote digestion and growth of fishes, were found to greatly improve the enzyme activities. In addition,the mix usage of M- I and M- E were proved to play a role in both regulating the wa ter quality and promoting digestion, which would further promote the growth and reproduction of grass carp. There fore , it could be concluded that microecologics were benefit for the rapid and healthy growth of grass carp farmed in the ponds.

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