南平烤烟GC-MS指纹图谱构建及模式识别

摘要

为了利用GC-MS指纹图谱技术对烤烟品质和风格特色进行质量控制,试验采用同时蒸馏萃取结合GC-MS方法构建了南平烤烟的致香物质标准指纹图谱,并选取了南平、云南、湖北和贵州烤烟样品对该标准指纹图谱进行分析,相似度计算结果表明,南平烤烟样品与该标准指纹图谱的相似度极高,判断的准确率在80％以上,而云南、湖北和贵州与该标准指纹图谱的相似度较低.这表明,南平烤烟与云南、湖北和贵州烟区烤烟的品质和风格特色具有明显差异.最后通过主成分投影图对南平、云南、湖北和贵州烟区烤烟的指纹图谱进行了模式识别,结果显示,南平烟区与其他烟区烤烟指纹图谱模式识别的差异明显,与相似度计算结果一致.综上表明,南平烤烟GC-MS指纹图谱具有较好可用性,能够用于控制南平烟区烤烟质量和特色风格的稳定性.%In order to test the quality and characteristics of flue-cured tobacco by the technology of GC-MS fingerprint, the method of SDE combined with GC-MS were used to construct a standard fingerprint about aroma constituent of the flue-cured tobacco from Nanping. Samples of leaves from 3 other areas ( Nanping, Yunnan, Hubei and Guizhou) were used to study the standard fingerprint. Results of similarity calculation showed that,flue-cured tobacco from Nanping had a much higher similarity with the standard fingerprint, the accuracy was more than 80% , while flue-cured tobacco from Yunnan,Hubei and Guizhou had a lower similarity. Compared with the ones from Yunnan, Hubei and Guizhou,flue-cured tobacco from Nanping showed marked difference in quality and characteristics. Pattern recognition on fingerprint was analyzed by PCA projection drawing of flue-cured tobacco from Nanping, Yunnan, Hubei and Guizhou. The result showed that,flue-cured tobacco from Nanping had significant difference in pattern recognition on fingerprint comparing with the ones from other 3 tobacco-planting areas, which was accordant with the result of similarity calculation. In conclusion,GC-MS fingerprint is feasible and can be used for the quality control and characteristics control of flue-cured tobacco from Nanping.