猪源乙型脑炎病毒河南分离株的全基因组测序及进化分析

摘要

为了解猪源乙型脑炎病毒(JEV)的全基因组结构及其分子进化规律,测定了3个JEV猪源分离株(BSF.ZZ-1、BSF.ZZ-3和CSF.XZ-2D)的全基因组序列,并对它们的氨基酸序列进行了分析.结果表明,CSF.XZ-2D全长为10 976 nt,而BSF.ZZ-1和BSF.ZZ-3全长均为10977 nt,在后2个毒株基因组的10701 nt位点处均有1个插入碱基“G”.BSF.ZZ-1、BSF.ZZ-3、CSF.XZ-2D与SA14株核苷酸和氨基酸同源性较高,核苷酸同源性都为99.4％,氨基酸同源性均为99.0％,与猪源分离株HW(HW1)、HWe(HW2)和SH0601的核苷酸与氨基酸同源性均为98.0％以上.用涵盖全部5个基因型的30个JEV毒株的全基因组序列构建系统进化树,发现这3个猪源分离株位于同一进化分支,均属于基因Ⅲ型.%In order to learn more about the complete genome and molecular evolution regular of Japanese encephalitis virus (JEV) strains isolated from swine, the full-length genome sequences of three JEV isolates, namely as BSF. ZZ-1 ,BSF. ZZ-3 and CSF. XZ-2D isolated from swine were analyzed in this paper. The results show that the length of the complete genomes of BSF. ZZ-1 ,BSF. ZZ-3 and CSF. XZ-2D was 10 977,10 977,10 976 nt,respectively. There is an insert base of " G" at the 10 701 nt site in the genomes of both BSF. ZZ-1 and BSF. ZZ-3. Compared to the virulent strain SA14 ,the homologies of nucleotide and amino acid sequences of BSF. ZZ-1 ,CSF. XZ-2D and BSF. ZZ-3 are 99. 4% and 99. 0% , respectively. Compared to the isolates HW ( HW1 ) , Hwe ( HW2 ) and SH0601 from swine,the homologies of the present three isolates are all more than 98. 0% . Phylogenetic analysises with other 30 reference isolates covering all five JEV genotypes indicated that BSF. ZZ-1 ,CSF. XZ-2D and BSF. ZZ-3 located in a same evolutionary branch,belonging to the genotype HI.