不同地区辣椒疫霉菌生理小种的鉴定和生物学特性的研究

摘要

为了确定不同地区采集的辣椒疫病病原菌的菌种类型及其生理小种类型，首先利用形态学和分子生物学的手段对广东、山西和内蒙古等地的辣椒疫病的病原菌进行了鉴定，结果表明，所分离到的7株病原菌均为辣椒疫霉菌。通过比较不同地区分离到的疫霉菌生物学特性，发现不同地区辣椒疫霉菌在菌落形态、孢子囊的数量、生长速度等方面存在显著的差异。利用国际通用的鉴别寄主，对分离到的7个辣椒疫霉菌进行生理小种的鉴定，结果表明，来源于广东的辣椒疫霉菌 P1是2号生理小种，而来源于山西和内蒙古的6株辣椒疫霉菌 P2~P7都是3号生理小种。目前，在山西和内蒙古地区并没有关于辣椒疫霉菌生理小种鉴定的任何报道，确定了这2个地区的辣椒疫霉菌的优势生理小种为3号生理小种。%In order to confirm the strain type and physiological race type of the pathogen causing Pepper blight and being collected from different regions,we utilized the morphological and molecular methods to identify the seven strains which were collected from Guangdong,Shanxi and Inner Mongolia as Phytophthora capsici Leonian. Through the comparison of biological characteristics of the seven strains from different regions,we found there were signifi-cant differences in colonial morphology,mycelial growth rate and sporulation ability. Through a set of the interna-tional differential hosts,we identified physiological race type of the seven strains,P1 which was from Guangdong be-longed to Race 2,P2 to P7 which were collected from Shanxi and Inner Mongolia all belonged to Race 3. At pres-ent,there is no report of the physiological race type of P. capsici in Shanxi and Inner Mongolia,our research′s results confirmed that the physiological race type of P. capsici in these two regions is Race 3. This results not only filled the vacancy in the research field of P. capsici,it would also lay a solid foundation for the resistance breeding against Pepper blight in the future.