首页> 中文期刊> 《华北农学报》 >大白菜抗根肿病基因位点CRa和CRb的分子标记鉴定

大白菜抗根肿病基因位点CRa和CRb的分子标记鉴定

         

摘要

利用大白菜抗根肿病基因 CRa和 CRb分子标记( SC2930和 KBrH129J18R)引物组,对78份大白菜材料进行抗根肿病分子标记鉴定。结果表明,在这78份材料中,有34份材料含有 SC2930-T( CRa抗病标记)标记,其中杂合抗病位点材料17份,纯合抗病位点材料17份。有37份材料含有 KBrH129J18R抗病标记,其中纯合抗病位点材料15份,杂合抗病位点材料22份。有20份材料不含有 CRa和 CRb所对应的抗病标记,23份材料含有2个抗病标记。该研究初步明确了78份参试大白菜材料所含抗根肿病基因 CRa和CRb类型,为大白菜抗根肿病育种提供材料选择依据。%The objective of this study was to use 2 gene molecular markers of clubroot resistance from Chinese cabbage(SC2930 and KBrH129J18R),to identify clubroot resistance genes in 78 resources of Chinese cabbage. The results showed that there were 34 resources with SC2930 resistance marker in 78 resources. Including 17 heterozygous loci materials and 17 homozygous loci materials. 37 resources had KBrH129J18R resistance marker. Including 22 het-erozygous loci materials and 15 homozygous loci materials. 20 resources hadn′t the resistance markers of CRa or CRb. 23 resources had both 2 resistance markers. The study defined the clubroot resistance gene types of CRa and CRb in the 78 resources,and provided a material basis of breeding for clubroot resistance in Chinese cabbage.

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