辣椒C.annuum×C.chinense种间杂种的获得及鉴定

摘要

种间杂交是种质创新和利用外源优异基因的重要途径。运用有性杂交方法，以浓紫色辣椒C．annuum PBC1366为母本（ P1）、强辣C．chinense PI439487为父本（ P2）进行种间杂交，获得了种间杂种植株。对杂种F1的19个表型性状进行了观察比较，结果表明，F1在生长势方面具有明显的杂种优势，其他表型性状介于父母本之间。种间F1花粉可染率为64．4％，形态学观察和EST-SSR标记分析结果明确了种间杂种F1的真实性。 C．annuum ×C．chinense种间杂种的获得为辣椒种间遗传图谱构建、辣椒香味和花青素代谢途径相关基因的定位奠定了基础。%Interspecific hybridization is the important way of excellent germplasm innovation and utilization of exogenous gene .With manual pollination ,an interspecific hybrid was obtained from the cross between thick purple pepper C.annuum PBC1366 as female parent ( P1 ) and strong spicy pepper C.chinense PI439487 as male parent . 19 phenotypic traits were observed and compared among P 1 ,F1 and P2 .The results showed that the F 1 had heterosis significantly and majority of the other phenotypic traits were intermediate to that of either parent .The pollen stain-ability percentages of interspecific F 1 was 64 .4%,the nature of hybrid was confirmed by morphological observation and EST-SSR marker analysis .The obtainment of the interspecific hybrid of C.annuum and C.chinense afford a basis for genetic map construction ,cocalization the fruit flavor gene ,and anthocyanins metabolic pathway gene in pepper .