紫苏脂肪酸硫酯酶基因PfFatA生物信息学及其表达特性分析

摘要

In order to detect the function of fatty acid thioesterase (FatA) in fatty acid synthesis mechansim of perilla, many bioinformatics analytical methods were used to analyze the protein encoded by FatA gene.The result showed that full length of cDNA for PfFatA was 1 652 bp,open reading frame(ORF)was 1 119 bp,coding 372 amino acids.FatA was an unstable hydrophilic protein,without transmembrane region.FatA protein contained a conserved domain of Acyl-ACP_TE,belonging to the Hotdog fold super protein family.Multiple sequence alignment indicated that FatA had highly conserved triple peptide of AKL,SKV in the C-terminal.Phylogenetic tree analysis showed that Perilla frutescens had the closest genetic relationship with Salvia miltiorrhiza.The result of Real-time PCR showed PfFatA was expressed in roots,stems,leaves,flowers and seeds from different development period,especially in seeds formed in 10 days after flowering.These results of the current study provided a foundation for further illustrating the function and mechanism of PfFatA gene.%为研究脂肪酸硫酯酶A(FatA)在紫苏脂肪酸合成机制中发挥的作用,对紫苏FatA基因及其编码的蛋白质进行生物信息学分析.结果表明,PfFatA基因cDNA全长1 652 bp,开放阅读框1 119 bp,编码372个氨基酸;紫苏FatA基因编码的蛋白质为亲水性不稳定蛋白,无跨膜区域;含Acyl-ACP_TE保守结构域,属于Hotdog fold超蛋白家族;多序列比对结果发现,序列C端含有保守的三联肽AKL和SKV结构;系统进化树分析表明,紫苏与丹参亲缘关系较近.通过Real-time PCR分析得知,PfFatA在紫苏根、茎、叶、花和种子不同发育时期均有表达,其中,在种子发育初期表达量最高.该研究结果可为进一步阐明PfFatA基因的功能及作用机制奠定基础.