紫外条件诱导下麦长管蚜DNA的变异研究

摘要

用RAPD-PCR技术研究了麦长管蚜Macrosiphum avenae在紫外辐射诱导下的DNA分子变异与多态性。用相似性指数（F）和遗传距离（D）及聚类分析对本研究所筛选的4种引物S88、S71、S103、S66的扩增结果分析表明：在紫外辐射（2×40w）诱导条件下蚜虫的平均遗传距离为0.2028，而在自然状态下蚜虫（对照）平均遗传距离为0.08125。紫外辐射处理下的实际遗传距离为0.12155，在该水平上DNA的变异频率为47.6667%。说明紫外辐射引起了蚜虫DNA的变异，该变异是否能遗传值得进一步研究。但足以说明紫外条件是引起蚜虫种下分化的主要因素之一。%The DNA mutation rate of Macroiphum avenae under the ultraviolet was studied by the method of random polymorphic amplified DNA (RAPD-PCR), and the method of cluster analysis based on the data of similarity index and Nei′s genetic distance. The result indicated that four primers(s88、s71、s103、s66) can be used for evaluating in the total clones, and that the average genetic distance equaled to 0.2028 under the ultraviolet (2×40 W), but it was 0.08125 under the normal condition. Therefore, the net genetic distance of the aphid under ultraviolet was 0.12155, and on this level, the rate of DNA mutation was about 47.6667%. Though we can conclude that the ultraviolet can result in mutation of the aphid DNA, whether this mutation has heredity remains to further study.