超声辅助法在亚洲日月蛤多糖提取工艺优化中的应用

摘要

目的：探讨超声辅助法对亚洲日月蛤多糖最佳提取工艺。方法：分别用热水浸提法和超声辅助热水浸提法提取亚洲日月蛤多糖，并比较两种方法的生物多糖得率。再分别以液固比、提取温度和提取时间为条件，采用超声辅助法提取亚洲日月蛤多糖，通过单因素正交实验确定多糖得率的最优提取条件。结果：相同条件下，热水浸提法提取出的亚洲日月蛤多糖得率为1．05％，而超声辅助热水浸提法的多糖得率为1．71％。超声辅助热水法提取亚洲日月蛤多糖的最优条件是：在超声功率160 W条件下，液固比40∶1（mL／g）、温度为90℃、时间4 h，在此条件下亚洲日月蛤多糖得率为2．01％，其中液固比（P＜0．01）和提取温度（ P＜0．05）对多糖提取率影响较大。结论：超声辅助法提取亚洲日月蛤多糖得率明显优于热水浸提法。%[Abstratc ]Objective:To optimize the ultrasound-assisted extraction technique in isolating the polysaccharide from Amusium pleuronce tes.Meth ods:Isola-tion of polysaccharide from Amusium pleuronetc es was performed with simple hot water extraction or ultrasound-assisted hot water extraction,and polysac-charide yield was compared pertaining to the two techniques .Then optimal polysaccharide extraction condition was determined by single factor and orthogo-nal test on water to material ratio,extraction temperature and extraction time basis.Results:Polysaccharide yield of Amusium pleuronectes was 1.05%by simple hot water extraction and 1.71%by ultrasound-assisted hot water isolation in the same condition .Optimal conditions for polysaccharide production by ultrasound assistance were as follows:ultrasound power at 160 W,water to material ratio by 40∶1(mL/g),extraction temperature at 90℃ and extraction time for 4 hours.This condition resulted in polysaccharide yield from Amusium pleuronectes by 2.01%.In addition,the water-material ratio and extraction time had great impact on the recovery rate( P<0.01;P<0.05,respectively) .Conclusion:Ultrasound-assisted hot water extraction may lead to higher poly-saccharide yield from Amusium pleuronectes than simple hot water isolation technique.