盐酸阿比朵尔体外抑制2009新型流感病毒A(H1N1)的作用

摘要

Objective To detect the in-vitro effects of arbidol hydrochloride against 2009 new influenza virus A (H1N1) . Methods The activity of arbidol hydrochloride against 2009 new influenza virus A (H1N1) was determined in MDCK cell cultures. Hemagglutination assay, observation of cytopathic effects, RT-PCR and quantitative RT-PCR tests were performed for determination of virus titers. Inhibition concentration 50% and cy-totoxic concentration 50% were calculated with Chou's Menu of Dose-Effect Program. Results Arbidol hydrochloride showed low cytotoxicity (cytotoxic concentration 50% > 100 μmol/L) and significant anti-2009 new influenza virus A (H1N1) activity in cell cultures. Inhibition concentration 50% were (5. 5 ±0. 9) , (3. 4 ±0. 8) , and (1. 5 ±0. 2) μmol/L in hemagglutination assay, cytopathic effect test, and quantitative RT-PCR assay, respectively. Conclusion Arbidol has low cytotoxicity and high anti-virus activity and can effectively trigger the activities of interferon and immune response, and therefore can be a valuable anti-influenza virus drug.%目的 检测盐酸阿比朵尔体外对2009新型流感病毒A(H1N1)复制的影响.方法 采用2009新型流感病毒A(H1N1)感染MDCK细胞系统,检测盐酸阿比朵尔抗流感病毒的活性.血凝实验、细胞病变保护实验、RT-PCR 和定量RT-PCR实验用于病毒滴度确定.使用Chou软件系统计算药物50%抑制病毒浓度和50%毒性浓度.结果 盐酸阿比朵尔在MDCK 细胞培养内显示毒性低(50%毒性浓度>100 μmol/L),且明显抑制2009新型流感病毒A(H1N1)的复制.在血凝实验、细胞病变保护实验和定量RT-PCR检测中,盐酸阿比朵尔50% 抑制病毒浓度分别为(5.5±0.9)、(3.4±0.8)和(1.5±0.2)μmol/L.结论 盐酸阿比朵尔明显抑制2009新型流感病毒A(H1N1)复制活性,而且具有诱生干扰素和免疫激活活性,作为一种抗流感病毒药物,它是有价值的.