富血小板纤维蛋白对粗糙钛板表面牙龈成纤维细胞增殖和分化的影响

摘要

Objective To study the effect of platelet rich fibrin (PRF) on proliferation and differentiation of human gingival fibroblasts on rough titanium surface. Methods Human gingival fibroblasts were cultured in vitro, and their fourth generation was co-cultured with rough titanium. The cells were then cultured in common medium and PRF medium respectively, and detected by MTT, RT-PCR and Western blot respectively. Results MTT showed that PRF could promote the proliferation of human gingival fibroblasts(P < 0.05). RT-PCR demonstrated that the expression level of typeⅠcollagen mRNA was higher than that of bFGF mRNA in PRF medium than in common medium(P<0.05). Western blot displayed that the expression level of typeⅠcollagen was higher in PRF medium than in common medium(P<0.05). Conclusion PRF can accelerate the proliferation and differentiation of human gingival fibroblasts on rough titanium surface.%　　目的探讨富血小板纤维蛋白对粗糙钛板表面人牙龈成纤维细胞增殖和分化的影响。方法体外培养人牙龈成纤维细胞，传至第4代与粗糙钛板联合培养用于实验。分别在普通培养基和含富血小板纤维蛋白的培养基中培养，MTT、RT-PCR、蛋白印迹技术进行检测。结果 MTT结果显示含加入富血小板纤维蛋白能明显促进粗糙钛板表面人牙龈成纤维细胞的增殖，差异有统计学意义(P＜0.05)。RT-PCR提示两组均有Type(Ⅰ)collagen和bFGF的mRNA表达，含富血小板纤维蛋白组mRNA表达比普通组明显增强。WB提示，Ⅰ型胶原蛋白表达在含富血小板纤维蛋白组中明显增强。结论富血小板纤维蛋白能促进粗糙钛板表面人牙龈成纤维细胞的增殖和分化。