Identification of transporter proteins for PQQ-secretion pathways by transcriptomics and proteomics analysis in Gluconobacter oxydans WSH-003

摘要

Pyrroloquinoline quinone (PQQ) plays a significant role as a redox cofactor in combination with dehydrogenases in bacteria.These dehydrogenases play key roles in the oxidation of important substrates for the biotechnology industry,such as vitamin C production.While biosynthesis of PQQ genes has been widely studied,PQQ-transport mechanisms remain unclear.Herein,we used both two-dimensional fluorescence-difference gel electrophoresis tandem mass spectrometry and RNA sequencing to investigate the effects ofpqqB overexpression in an industrial strain of Gluconobacter oxydans WSH-003.We have identified 73 differentially expressed proteins and 99 differentially expressed genes,a majority of which are related to oxidation-reduction and transport processes by gene ontology analysis.We also described several putative candidate effectors that responded to increased PQQ levels resulting from pqqB overexpression.Furthermore,quantitative PCR was used to verify five putative PQQ-transport genes among different PQQ producing strains,and the results showed that ompW,B932_ 1930 and B932_2186 were upregulated in all conditions.Then the three genes were over-expressed in G.oxydans WSH-003 and PQQ production were detected.The results showed that extracellular PQQ of B932_1930 (a transporter) and B932_2186 (an ABC transporter permease) overexpression strains were enhanced by 1.77-fold and 1.67-fold,respectively.The results suggest that the proteins encoded by PqqB,B932_1930 and B932_2186 might enhance the PQQ secretion process.