声明
总结
Chapter 1 Introduction
1.1 Background
1.1.1 Energy crises
1.1.2 Global warming and climate change
1.1.3 Water pollution
1.1.4 Sustainable development
1.2 Biofuel as energy feedstock
1.3 Microalgae as energy feedstock
1.4 Microalgae cultivation technology
1.4.1 Microalgae biology
1.4.2 Culture parameters
1.4.3 Algae biomass harvesting techniques
1.5 Down streaming processing
1.5.1 Microalgae lipid extraction
1.5.2 Microalgal biodiesel conversion
1.5Research objective
Chapter 2 Materials and methods
2.1 Microalgae strain and culture medium
2.2 Main chemical reagent and laboratory apparatus
2.3 Culture condition and inoculums preparation
2.4 Experimental setup
2.5 Analysis of samples
2.5.1 Analysis of growth parameters
2.5.2 pH measurement
2.5.3 Nile red staining
2.5.4 Nile red fluorescent measurement DMSO concentration
2.5.5 Folch method
2.5.6 Determination of carbohydrates concentration
2.5.7 Determination of protein concentration
2.5.8 Fatty acids analysis
Chapter 3 Results and discussion
3.2 Biochemical response to bicarbonate supplementation on biomass and lipid productivity of Chlorella sorokiniana
3.3 Biochemical response combined supplementation ofsodium bicarbonate and CO2 on biomass and lipid productivity of Chlorella sorokiniana
Chapter 4 Conclusion
参考文献
Academic Achievements and Participation in Scientific Research
致谢
天津大学;
