Chapter 1 Reviewof the literature
1.1Overview of Elaeagnus mollis oil
1.1.1 Overview of Elaeagnus mollis Diels
1.1.2Nutrients of Elaeagnus mollis oil
1.1.3Health function of Elaeagnus mollis oil
1.2Overview of non-alcoholic fatty liver disease
1.2.1Pathogenesis of non-alcoholic fatty liver disease
1.2.2Therapeutic method of non-alcoholic fatty liver disease
1.3The significance and main content of the study
1.3.1The significance of the study
1.3.2Main content of the study
Chapter 2 Effects of Elaeagnus mollis oil on basic indicators in high-fat diet induced mice
2.1 Introduction
2.2 Materials and methods
2.2.1 Materials
2.2.2 Animal protocol
2.2.3Collection and preparation of samples from experimental animal
2.2.4Serum lipids analyses
2.2.6Statistical analyses
2.3 Results
2.3.1Effect of Elaeagnus mollis oil on food intake in high-fat diet induced mice
2.3.2Effect of Elaeagnus mollis oil on food efficiency ratioin high-fat diet induced mice
2.3.3Effect of Elaeagnus mollis oil on energy intakein high-fat diet induced mice
2.3.4Effect of Elaeagnus mollis oil on body weightin high-fat diet induced mice
2.3.5Effect of Elaeagnus mollis oil on fat weight and fat coefficientin high-fat diet induced obsess mice
2.3.6Effect of Elaeagnus mollis oil on Lee’s indexin high-fat diet induced mice
2.3.7Effect of Elaeagnus mollis oil serum lipidsin high-fat diet induced mice
2.3.8Evaluation of histopathological sections
2.3.9 Correlation analysis
2.4 Discussion
2.5 Conclusions
Chapter 3 Effects of Elaeagnus mollis oil on hepatic lipids accumulation, hepatic injury and hepatic oxidation stress in high-fat diet induced mice
3.1 Introduction
3.2 Materials and methods
3.2.1 Materials
3.2.2Animal protocol
3.2.3Collection and preparation of samples from experimental animal
3.2.4Serum biochemical analyses
3.2.5Measurement of hepatic profiles
3.2.6Analyses of antioxidant capacity
3.2.7 Histopathology
3.2.8Statistical analyses
3.3.1Effect of Elaeagnus mollis oil on liver weight andliver coefficient in high-fat diet induced mice
3.3.2Evaluation of hepatic histopathological sections
3.3.3Effect of Elaeagnus mollis oil on hepatic lipidsin high-fat diet induced mice
3.3.4Effect of Elaeagnus mollis oil on hepatic injuryin high-fat diet induced mice
3.3.5Effect of Elaeagnus mollis oil on hepatic oxidation stress in high-fat diet induced mice
3.3.6 Correlation analysis
3.4 Discussion
3.5 Conclusions
Chapter 4 Effects of Elaeagnus mollis oil on hepatic lipids metabolism related mechanisms in high-fat diet induced mice
4.1 Introduction
4.2 Materials and methods
4.2.1 Materials
4.2.2Animal protocol
4.2.3Collection and preparation of samples fromexperimental animal
4.2.5 Reverse transcription
4.2.6 Quantitative real-time PCR (qRT-PCR)
4.2.7Statistical analyses
4.3.1 Effect of Elaeagnus mollis oil on SREBP-1c gene expression in high-fat diet induced mice
4.3.2Effect of Elaeagnus mollis oil on PPARγ gene expression in high-fat diet induced mice
4.3.3 Effect of Elaeagnus mollis oil on FAS gene expression in high-fat diet induced mice
4.3.4Effect of Elaeagnus mollis oil on ACC gene expression in high-fat diet induced mice
4.3.5Effect of Elaeagnus mollis oil on PPARα gene expression in high-fat diet induced mice
4.3.6Effect of Elaeagnus mollis oil on CPT-1 gene expression in high-fat diet induced mice
4.3.7Effect of Elaeagnus mollis oil on ACOX gene expression in high-fat diet induced mice
4.3.8 Correlation analysis
4.4 Discussion
4.5 Conclusions
Chapter 5 Effects of Elaeagnus mollis oil on amounts and compositions of fatty acid in liver
5.1 Introduction
5.2Materials and methods
5.2.1 Materials
5.2.2 Animal protocol
5.2.3 Collection and preparation of samples fromexperimental animal
5.2.4 Extraction methylation of the fatty acids
5.2.5Methylation of the fatty acids
5.2.6Analysis of fatty acids by GC-MS
5.2.7Statistical analyses
5.3 Results
5.3.1 Effect of Elaeagnus mollis oil on the compositions and contents of fatty acids in liver
5.3.2 Effect of Elaeagnus mollis oil on the unsaturated degree in liver
5.4 Discussion
5.5 Conclusions
Chapter 6 Conclusion and future work
6.1 Conclusions
6.2 Future work
参考文献
Appendix
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