绞股蓝提取物对鼠成纤维细胞作用的研究

摘要

背景:本文旨在证明绞股蓝提取物对小鼠皮肤成纤维细胞的作用。近期研究表明绞股蓝具有强大的抗氧化能力，这一特点使其适于对抗氧化压力。方法:制备绞股蓝提取物，培养小鼠皮肤成纤维细胞。在相同条件下培养12组细胞，同时设立阴性和阳性对照组。UVC照射产生氧化压力，显微镜下进行活细胞计数，用SPSS软件进行数据分析。结果:绞股蓝的浓度与细胞的平均生存时间呈正相关(rs=1)，具有统计学意义(P＜0.01)。同时，三次试验之间存在显著正相关(rs=0.988-1，P＜0.01)。结论:绞股蓝提取物可以延长经UVC照射的小鼠成纤维细胞的寿命，在对抗皮肤细胞老化方面有潜在的优势。

目录

声明

摘要

Abstract

Contents

1 ORIENTATION OF THE STUDY

1．1 INTRODUCTION

1．2 RATIONALE

1．3 PROBLEM STATEMENT

1．4 RESEARCH QUESTION

1．5 AIM OF THE STUDY

1．6 OBJECTlVES

1．7 RESEARCH METHODOLOGY

1．8 DEFINITIONS

1．9 SUMMARY

2 LITERATURE REVIEW

2．1 INTRODUCTION

2．2 Effects of external agents on skin aging

2．3 The action of external agents on the synthesis of collagen and the regulation of MMPs

2．4 Effects of internal agents on skin aging

2．5 Gynostemma pentaphyllum as an antioxidant of interest

2．6 Scientific studies asserting the antioxidant properties of Gynostemma pentaphyllum

2．7 Scientific studies asserting other useful properties of Gynostemma pentaphyllum

2．8 SUMMARY

3 METHODOLOGY

3．2 INTRODUCTION

3．3 THE PURPOSE OF THE STUDY

3．4 THE RESEARCH OBJECTIVES

3．5 THE RESEARCH DESIGN

3．5．1 Qruantitative research

3．5．2 Exploratory descriptive design

3．5．3 Qualitative research

3．6 RESEARCH SETTING

3．7 STUDY

3．8 MATERIALS AND METHODS

3．9 ROLE OF THE RESEARCHER

3．10 ETHICAL CONSIDERATIONS

3．11 DISSEMINATION OF RESULTS

3．12 LIMITATION OF THIS STUDY

3．13 SUMMARY

4 ANALYSIS AND PRESENTATION OF THE RESULTS

4．1 INTRODUCTION

4．2 SUMMARY

4．3 Induction of oxidative stress with the use of ultraviolet light C

4．4 Qualitative analysis of cell viability following the administration of Gynostemma pentaphyllum

4．5 Correlation between the concentration of Gynostemma pentaphyllum and cell viability

4．6 Correlation between independent trials

5 DISCUSSION AND RECOMMENDATIONS

5．1 INTRODUCTION

5．2 DISCUSSION

5．3 BIAS AND LIMITATIONS

5．4 CONCLUSION AND RECOMMENDATIONS

5．5 CONCLUDING REMARK

ACKNOWLEDGEMENTS

REFERENCES