Packed-Bed Reactor Running on Babassu Oil and Glycerol to Produce Monoglycerides by Enzymatic Route using Immobilized Burkholderia cepacia Lipase

摘要

The aim of this study was the glycerolysis of babassu oil catalyzed by immobilizedlipase from Burkholderia cepacia, in a continuous packed-bed reactor. The best reactionconditions were previously established in batchwise via response surface methodology as afunction of glycerol-to-oil molar ratio and reaction temperature. The reactor operatedcontinuously for 22 days at 50 °C, and during the first 6 days, no significant decrease on theinitial lipase activity was observed. Monoglycerides concentration was in the range from 25 to33 wt.%. Subsequently, a progressive decrease in the activity was detected, and an inactivationprofile described by Arrhenius model estimated values of 50 days and 1.37×10?2h?1, for thehalf-life and deactivation coefficient, respectively.