【24h】

AOTF-based system for image cytometry

机译:基于AOTF的图像细胞计数系统

获取原文
获取原文并翻译 | 示例

摘要

This paper describes a simple, inexpensive multispectral imaging system for image cytometry applications. The system is based on an acousto-optical tunable filter (AOTF), a monochrome CCD camera, and a research-grade fluorescence microscope. The selected TeO_2 AOTF has a 10 x 10 mm~2 entrance aperture and operates within the spectral range of 447-750 nm. The bandpass of the filter varies between 1.4 nm at 450 nm and 5.1 nm at 690 nm. The control software works within the environment of a popular image-acquisition and -processing package Image Pro-Plus, making this system easy to integrate with many existing fluorescence microscopes and cameras. Since image-cytometry applications do not require very high spatial resolution, AOTF-based systems may become an interesting alternative to more complex and expensive LCTF or pushbroom methods.
机译:本文介绍了一种简单,便宜的多光谱成像系统,适用于图像细胞分析应用。该系统基于声光可调滤光片(AOTF),单色CCD相机和研究级荧光显微镜。所选的TeO_2 AOTF具有10 x 10 mm〜2的入射孔径,并在447-750 nm的光谱范围内工作。滤光片的带通在450 nm的1.4 nm和690 nm的5.1 nm之间变化。该控制软件可在流行的图像采集和处理软件包Image Pro-Plus的环境中运行,从而使该系统易于与许多现有的荧光显微镜和照相机集成。由于图像细胞计数法的应用不需要很高的空间分辨率,因此基于AOTF的系统可能成为更复杂,更昂贵的LCTF或推扫方法的有趣替代方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

联系方式:18141920177 (微信同号)

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号