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AOTF-based system for image cytometry

机译:基于AOTF的图像细胞计数系统

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This paper describes a simple, inexpensive multispectral imaging system for image cytometry applications. The systemis based on an acousto-optical tunable filter (AOTF), a monochrome CCD camera, and a research-grade fluorescencemicroscope. The selected TeO2 AOTF has a 10x10 mm~2 entrance aperture and operates within the spectral range of447–750 nm. The bandpass of the filter varies between 1.4 nm at 450 nm and 5.1 nm at 690 nm. The control softwareworks within the environment of a popular image-acquisition and -processing package Image Pro-Plus, making thissystem easy to integrate with many existing fluorescence microscopes and cameras. Since image-cytometry applicationsdo not require very high spatial resolution, AOTF-based systems may become an interesting alternative to morecomplex and expensive LCTF or pushbroom methods.
机译:本文介绍了一种简单,便宜的多光谱成像系统,适用于图像细胞分析应用。该系统基于声光可调滤光片(AOTF),单色CCD相机和研究级荧光显微镜。所选的TeO2 AOTF具有10x10 mm〜2的入射孔径,并在447–750 nm的光谱范围内工作。滤光片的带通在450 nm的1.4 nm和690 nm的5.1 nm之间变化。控制软件在流行的图像采集和处理软件包Image Pro-Plus的环境中工作,使该系统易于与许多现有的荧光显微镜和照相机集成。由于图像细胞计数法的应用不需要很高的空间分辨率,因此基于AOTF的系统可能成为更复杂,更昂贵的LCTF或推扫方法的有趣替代方法。

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