Cultivation of Rat Nerve Cells on Nanoimprinted Microstructures on Polydimethylsiloxane Sheets

摘要

Regenerative medical research has attracted much attention in recent years. Here, we fabricated micro trench structures with varying widths of 15 and 150 μm and depths of 30 and 60 μm using the nanoimprint technique on a 100 μm thick polydimethylsiloxane culture sheet used as a biocompatible polymer culture sheet. Rat neurons were observed to grow after 3 days selectively within those micro trenches with widths larger than 15 μm, and they were coated with polylysine.